Phylogenetic analysis based on 16S rRNA gene of a thermophilic protease-secreting Bacillus gelatini-TPNK-3 isolate from Kiteezi Landfill, Uganda.
John Omara, Joseph Fuuna Hawumba, Tobius Papa Ekeya Pol J Microbiol 2012; 61 (3): ICID: 1014501 Article type: Short communication IC™ Value: 6.00
Abstract provided by Publisher
A thermophilic protease-secreting bacterial isolate, TPNK-3, from Kiteezi landfill, is an aerobic Gram-positive spore-forming bacterium with rod-shaped cells (3.28 µm long and 0.45 µm wide). It grows optimally at 55 0C and pH of 7.0, tolerates up to 5 % (w/v) NaCl, exhibits extracellular amylolytic, cellulolytic and caseinolytic activities, utilizes a range of carbon and nitrogen sources and has a GC content of 45 mol %. The 16S rRNA gene sequence analysis showed it belongs to the genus Bacillus, and closest to Bacillus gelatini (> 99.9 %). Consequently, isolate TPNK3 is tentatively described as Bacillus gelatini strain TPNK3.