Use of the xCelligence system for purposes of medical microbiology

Adam Feliks Junka, Adriana Janczura, Danuta Smutnicka, Beata Mączyńska, Secewicz Anna, Joanna Nowicka, Marzenna Bartoszewicz, Grażyna Gościniak
Pol J Microbiol
2012; 61 (3):
ICID: 1014506
Article type: Original article
IC™ Value: 10.00
Abstract provided by Publisher
 
The real-time measurement of electrical impedance focuses increasing attention of microbiologists. Roche’s xCelligence impedance-measuring instrument is one of a few commercially available systems of such type. According to the best knowledge of authors, instrument was tested only for eukaryotic cells research as far.
Purpose: The aim of this work was to estimate xCELLigence suitability for the microbiological tests, including measurement of morphological changes in eukaryotic cells as a result of bacterial toxin activity, measurement of bacterial biofilm formation and impact of antiseptic on the biofilm structure.
Materials/Methods: To test the influence of bacterial LT enterotoxin on eukaryotic cell lines, Chinese Hamster Ovary cells and reference Escherichia coli ATTC 35401 strain were used; to investigate Roche’s instrument ability to measure biofilm formation and antiseptic impact on it, S.aureus ATTC6538 reference strain was used.
Results: Obtained data indicate excellent ability of xCelligence instrument to detect cytopathic effect caused by bacterial LT endotoxin, system was also able to detect staphylococcal biofilm formation. However, interpretation of results obtained during real–time measurement of antiseptic’s bactericidal activity against staphylococcal biofilm, causes many difficulties.
Conclusion: xCelligence instrument can be used for real–time monitoring of morphological changes in CHO cells treated with bacterial LT enterotoxin and for real–time measurement of staphylococcal biofilm formation in vitro. Further investigation is necessary to confirm suitability of system to analyze antiseptic’s antimicrobial activity against biofilm in vitro.
Key words: microbiology, impedance, biofilm, real-time measurement.

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