Study on regulation of growth and biosynthesis of cellulolytic enzymes from newly isolated Aspergillus fumigatus ABK9

Arpan Das, Tanmay Paul, Suman Kumar Halder, Pradeep Kumar Das Mohapatra, Bikash Ranjan Pati , Keshab Chandra Mondal
Pol J Microbiol
2013; 62 (1):
ICID: 1039005
Article type: Original article
IC™ Value: 10.00
Abstract provided by Publisher
 
This study was aimed to evaluate the pattern of cellulase biosynthesis from Aspergillus fumigatus ABK9 under submerged fermentation. Production was increased concomitantly with fungal growth upto 72h and reached maximum (Xmax – 6.72 gl-1) with specific growth rate (µmax) of 0.126 h-1. Highest specific rate of enzyme production (qp) was found at initial medium pH of 5.0 and incubation temperature of 30°C. At the same time, in presence of 2-deoxy-glucose concentration of 0.5 mg/ml, the production of cellulolytic enzymes, viz, carboxymethyl cellulase activity (CMCase), filter paper degrading activity (FPase) and β-glucosidase activity reached maximum up to 132.2, 21.3 and 28.9 Uml-1 respectively. Cellulase biosynthesis was induced in respect to higher volumetric production rate (Qp), specific rate of enzymes production (qp, Ug-1 biomass h-1) and enzyme/biomass yield (YE/X) when grown in carboxymethyl cellulose in comparison to other saccharides as sole carbon source. Induction ratios (IR) of cellulases were between 12.3 and 24.4 in presence of 1.5% (w/v) CMC in the culture media. The strain was quite resistant to catabolic repression by glucose upto 0.4% (w/v). Cellulases production was greatly influenced in presence of yeast extract and KH2PO4 as nitrogen and phosphate source in the culture media. C/N ratio of 10.0 and C/P ratio of 4.0 were proved to be the best for the production of enzyme cocktail. Along with the high production yield, the crude enzymes showed a promising cellulose hydrolyzing efficiency of rice straw, indicating the enzyme could be beneficial for its large scale industrial exploitation.
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