Efficiency of selected mutagens in generating Xanthophyllomyces dendrorhous strains hyperproducing astaxanthin
Barbara Stachowiak Pol J Microbiol 2013; 62 (1): ICID: 1039009 Article type: Original article IC™ Value: 10.00
Abstract provided by Publisher
The aim of the study was to assess the efficiency of selected mutagens: UV radiation, benomyl, ethyl methanesulfonate and ethidium bromide in generating asthaxanthin-hyperproducing strains of the yeast Xanthophyllomyces dendrorhous DSM 5626. It was subjected to mutations using the above mentioned mutagens. Next screening was performed on the obtained mutants to isolate strains hyperproducing astaxanthin. The criterion was the colour of colonies grown on a solid medium: intense pink to claret-coloured. Cell concentration, total carotenoid contents and astaxanthin yields were assessed in liquid media.
Asthaxanthin-hyperproducing strains were obtained as a result of mutation with benomyl, ethidium bromide and UV radiation. Ethyl methanesulfonate turned out to be useless in this respect. Mutations with benomyl generated the most numerous group of hyperpigmented mutants exhibiting increases up to 100% in astaxanthin contents.