Application of FLiP method for differentiation of Mycobacterium tuberculosis strains in comparison to commonly used methods, spoligotyping and MIRU-VNTR typing.
Anna Zaczek, Anna Brzostek, Alina Gorna, Anna Sajduda, Jaroslaw Dziadek Pol J Microbiol 2013; 62 (1): ICID: 1039010 Article type: Short communication IC™ Value: 6.00
Abstract provided by Publisher
The current "gold standard" in molecular epidemiological studies of Mycobacterium tuberculosis is IS6110 RFLP based on the IS6110 polymorphism. Recently, PCR-based methods are becoming increasingly important. Newly, fast ligation-mediated PCR (FLiP), based on the IS6110 polymorphism, was proposed. In this study, the discriminatory power of FLiP, spoligotyping and MIRU-VNTR typing, in differentiation of M. tuberculosis isolates was compared. The discriminatory index (HGI) of spoligotyping, MIRU-VNTR analysis, and FLiP was 0.653, 0.837, and 0.917, respectively. This indicates that FLiP allows a high level of differentiation among M. tuberculosis strains and it might be a valuable alternative for the other typing methods.