New PCR test for detection of Candida glabrata based on the molecular target chosen by the RAPD technique
Anna Olchawa, Beata Krawczyk, Anna Brillowska-Dąbrowska Pol J Microbiol 2013; 62 (1): ICID: 1039012 Article type: Original article IC™ Value: 10.00
Abstract provided by Publisher
Rapid and reliable diagnosis is a necessary condition for the rational and successful treatment of infections. Such diagnostic methods and assays are continually being developed. One important factor is the selection of the correct molecular targets. The paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of the RAPD patterns obtained with the DNA of microorganisms which could be present in the specimens. Sequence encoding Candida glabrata CBS138 hypothetical protein (CAGL0M05005g) was selected and the specificity of the designed primers complementary to this sequence was confirmed. The limit of detection for optimised PCR and real-time PCR reactions with DNA extracted from blood samples spiked with Candida glabrata was estimated at 1 CFU/ml. The application of the assays developed in this study would thus seem to be promising as the complementary method in diagnostics of C. glabrata infections.