Adherence of Group B Streptococci to Human Rectal and Vaginal Epithelial Cell Lines in relation to the Capsular Polysaccharides as well as Alpha-like Protein Genes – Pilot Study.

Małgorzata Bodaszewska-Lubaś, Monika Brzychczy-Włoch, Paweł Adamski, Tomasz Gosiewski, Piotr B Heczko
Pol J Microbiol
2013; 62 (1):
ICID: 1039013
Article type: Original article
IC™ Value: 10.00
Abstract provided by Publisher
 
Streptococcus agalactiae (Group B Streptococci, GBS) constitutes a risk factor for infections of
the newborns born by colonized mothers. The adherence of GBS to epithelial
cells has been proved to be an important factor in the colonization of mucus
membranes of both human rectum and vagina.

The objective of the
study was to assess the adhesion of the selected GBS
strains to the human colon adenocarcinoma cell line (HT-29) and human
epidermoid vulvo-vaginal cells (A-431) in
relation to the capsular polysaccharides and alpha-like protein genes.

GBS strains from the human
sources belonging to Ia, Ib, II, III or V serotypes possessing different alpha-like
protein genes such as the alp2, alp3, bca, epsilon or rib in the conventional adherence assay
were examined.

The adherence of GBS strains to the HT-29
cell line was considerably higher than to the A-431 cell line. For GBS serotype
Ia and III, a significant difference
between the adhesion to the HT-29 and A‑431 cell lines was presented. The adhesion
of GBS strains to the HT-29 cell line depended on alpha-like protein genes. The
most adhesive ones were the GBS strains containing the rib and alp2 genes. The
adherence of GBS strains to the A-431 cell line depended on both their serotype
and alpha-like protein genes. Serotype III adhered to the A-431 cells most
tightly, particularly the strains containing the rib
and alp2 genes. GBS strains
containing the rib gene adhered to the
HT-29 and A-431 cell lines more firmly than GBS strains containing other alpha-like
protein genes.
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