The Detection of Enteroviruses in Sewage Using Caco-2 Cells.

Magdalena Wieczorek, Łukasz Kuryk, Agnieszka Witek, Anna Diuwe, Bogumiła Litwińska
Pol J Microbiol
2013; 62 (1):
ICID: 1039015
Article type: Short communication
IC™ Value: 6.00
Abstract provided by Publisher
 
The work presented here demonstrates the utility of Caco-2 cells to detect enteroviruses in sewage. Viruses were concentrated by beef extract elution and organic flocculation prior to analysis by cell culture assays and RT-PCR. Enteroviruses were detected in all sewage samples, but only one sample was positive solely in RT-PCR assay. We proved that Caco-2 cells were more effective than RD and L20B cells in enterovirus isolation, depending on procedures used in inoculation process.
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