The Detection of Enteroviruses in Sewage Using Caco-2 Cells.
Magdalena Wieczorek, Łukasz Kuryk, Agnieszka Witek, Anna Diuwe, Bogumiła Litwińska Pol J Microbiol 2013; 62 (1): ICID: 1039015 Article type: Short communication IC™ Value: 6.00
Abstract provided by Publisher
The work presented here demonstrates the utility of Caco-2 cells to detect enteroviruses in sewage. Viruses were concentrated by beef extract elution and organic flocculation prior to analysis by cell culture assays and RT-PCR. Enteroviruses were detected in all sewage samples, but only one sample was positive solely in RT-PCR assay. We proved that Caco-2 cells were more effective than RD and L20B cells in enterovirus isolation, depending on procedures used in inoculation process.