Bifidogenic and antioxidant activity of exopolysaccharides produced by Lactobacillus rhamnosus E/N on different carbon sources
Magdalena Polak-Berecka, Adam Waśko, Dominik Szwajgier, Adam Choma Pol J Microbiol 2013; 62 (2): ICID: 1055711 Article type: Original article IC™ Value: 10.00
Abstract provided by Publisher
Lactobacillus rhamnosus E/N is a probiotic bacterium which synthesizes exopolysaccharides (EPS) with significant bifidogenic and antioxidant activities. This bacterium produces EPS from respective carbohydrate sources in the growth medium (EPS-Glc, EPS-Lac, EPS-Gal, EPS-Mal, and EPS-Suc). These exopolysaccharides are composed of rhamnose, glucose, mannose, and galactose in amounts dependent on the kind of carbon source in the growth medium. Five Bifidobacterium strains (B. infantis ATCC1567, B. bifidum B 41410, B. animalis B41406, B. animalis BI30, and B. longum KN 29) were tested in vitro for their ability to utilize all EPSs studied. The highest bifidogenic activity was shown by EPS-Gal, EPS-Lac, and EPS-Mal. The antioxidant and free radical scavenging potentials of the isolated EPSs were studied using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method. In the methods with ABTS/myoglobin and ABTS/potassium persulphate, a significant antioxidant effect was observed for EPS-Gal, EPS-Lac, and EPS-Suc.