Tissue Tropism of Ornithobacterium rhinotracheale in Chickens Determined by Culture and Nucleic Acid Detection
Artur Żbikowski, Piotr Szeleszczuk , Marian Binek Pol J Microbiol 2013; 62 (2): ICID: 1055715 Article type: Short communication IC™ Value: 6.00
Abstract provided by Publisher
Although several years have passed since the discovery and classification of the etiological factor of ornithobacteriosis the disease is still prevalent causing significant economical losses in poultry industry. The aim of the present study was to evaluate the ability of Ornithobacterium rhinotracheale (ORT) to colonize chosen organs of chicks infected intratracheally (group A1), or intravenously (group A2), with the use of bacteriological methods and PCR. The bacteriological methods enabled to reisolate ORT bacteria from trachea and lungs of the birds from group A1 only on day 3 and 6 after infection. The PCR technique additionally detected the bacterial genetic material in these organs on the 9th day after infection, and gave positive results in the samples from air sacs until the 6th day of experiment. In birds infected intravenously (A2) ORT was reisolated from liver on day 3 and from spleen on day 3 and 6 after infection, whereas the reisolation from the air sacs occurred during the entire experimental period. PCR enabled to detect the bacterial DNA in the liver, spleen and lungs of chickens until the 9th day after infection and in case of air sacs during the whole time of the study.