Serological studies of Proteus penneri strains determininga qualification to an appropriate O-serogroup
Agata Palusiak, Małgorzta Siwińska, Zygmunt Sidorczyk Pol J Microbiol 2013; 62 (2): ICID: 1055716 Article type: Short communication IC™ Value: 6.00
Abstract provided by Publisher
At first the Proteus classification scheme contained strains only from two Proteus species: P. mirabilis and P. vulgaris. Describing a new species of P. penneri resulted in the necessity of its classification. The Department of General Microbiology created a wide collection of P. penneri isolates coming from different parts of the world. 116 P. penneri strains from this collection were classified into 19 different O-serogroups. This work contains the classification of 12 remaining P. penneri strains. The lipopolysaccharides (LPSs) extracted from P. penneri strains were tested in an enzyme-linked immunosorbent assay (ELISA) with selected O-antisera against Proteus sp. strains representing all defined Proteus sp. O-serogroups. Homologous and cross-reacting systems were checked in: passive immunohemolysis (PIH), inhibition of ELISA and PIH and Western blot procedure. 11 cross-reacting systems observed in the preliminary studies were selected for detailed serological studies. The PIH results with absorbed antisera proved the identity of LPSs within each tested system. Two antisera appeared to contain small amounts of O-polysaccharide-specific antibodies. Comparing the results of all serological studies led to the classification of twelve tested P. penneri strains into five different Proteus sp. O-serogroups, thus completing the serological classification of the unique collection of P. penneri strains.