The influence of toll-like receptor stimulation of EBV lytic genes
Joanna Siennicka, Agnieszka Trzcińska, Agnieszka Częścik, Milena Dunal-Szczepaniak, Barbara Łagosz Pol J Microbiol 2013; 62 (3): ICID: 1072788 Article type: Original article IC™ Value: 10.00
Abstract provided by Publisher
EBV establishes latency in the resting memory B-cell compartment. It has been recently suggested that maintenance of chronic infection is dependent on periodic reactivation. Although the stimuli for EBV reactivation in vivo during natural infections are largely unknown, there is evidence indicating that heterologous infections could trigger herpesviruses reactivation. The purpose of this work was to identify the influence of Toll-like receptors stimulation on EBV replication in EBV latently infected Burkitt lymphoma cells (P3HR-1, Raji and Namalwa). The cells were stimulated with Pam3CSK4, PolyI:C, LPS, measles virus (MeV) and PMA. Non-stimulated cells (NS) cells served as control. EBV expression was investigated at m-RNA level for three viral lytic genes: BZLF1, BALF2 and BcLF1. Additionally, the effect of stimulation on NF-kBp65 and inflammatory cytokines (IL-1b, IL-6, IL-8, IL-10, IL-12p70, and TNF) was investigated. Stimulation of TLRs led to limited changes in EBV expression manifesting as increase of ZEBRA at m-RNA level in cells treated with PolyI:C and Pam3CSK4. Stimulation with PolyI:C, Pam3CSK4 and LPS also lead to considerable increase of NF-kB, while the increase levels of inflammatory cytokines were observed for IL-8, TNF and IL-6 in cells treated with PMA and MeV. In conclusion, results of our experiment support suggestion that TLRs stimulation could influence on EBV virus replication.