Salt stress tolerance of methylotrophic bacteria Methylophilus sp. and Methylobacterium sp. isolated from coal mine spoils

Deen Dayal Giri, Ajay Kumar, Prabhu Nath Shukla, Ritu Singh, Kapil Deo Pandey
Pol J Microbiol
2013; 62 (3):
ICID: 1072792
Article type: Original article
IC™ Value: 10.00
Abstract provided by Publisher
 
Two methylotrophic strains of Bina coalmine spoil BNV7b and BRV25 based on physiological traits and 16S rDNA sequence identified as Methylophilus and Methylobacterium species. The strains exhibited similar carbon utilization but differed in N utilization and their response to the metabolic inhibitors. Methylophilus sp. was less tolerant to salt stress and it viability declined to one tenth within 4h of incubation in 2M NaCl due to membrane damage and leakage of the intracellular electrolytes as evident from malondiaaldehyde (MDA) assay.  In 200mM NaCl, they exhibited increased superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity while in 500mM NaCl, enzyme activities declined in Methylophilus sp. and increased in Methylobacterium sp. Among exogenously applied osmoprotectants proline was most efficient; however, polyols (mannitol, sorbitol and glycerol) also supported growth under lethal NaCl concentration.
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