Identification of Scopulariopsis Species by Partial 28S rRNA Gene Sequence Analysis

Tomasz Jagielski, Kinga Kosim, Magdalena Skóra, Anna Barbara Macura, Jacek Bielecki
Pol J Microbiol
2013; 62 (3):
ICID: 1072797
Article type: Short communication
IC™ Value: 6.00
Abstract provided by Publisher
 
The genus Scopulariopsis contains over 30 species of mitosporic moulds, which although usually saprophytic may also act as opportunistic pathogens in humans. They have mainly been associated with onychomycosis, and only sporadically as a cause of deep tissue infections or systemic disease. Identification of Scopulariopsis species still largely relies on phenotype-based methods. There is a need for a molecular diagnostic approach, that would allow to reliably discriminate between Scopulariopsis species. The aim of this study was to apply sequence analysis of partial 28S rRNA gene for species identification of Scopulariopsis clinical isolates. Although the method employed did reveal some genetic polymorphism among Scopulariopsis isolates tested, it was not enough for species delineation. For this to be achieved, other genetic loci, within and beyond the rDNA operon, need to be investigated.
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