Report on overcoming the poor quality of ApaI pulsotypes with a short review on PFGE for Listeria monocytogenes
DARJA KUŠAR, MAJA KAVALIČ, MATJAŽ OCEPEK, IRENA ZDOVC Pol J Microbiol 2013; 62 (3): ICID: 1072798 Article type: Short communication IC™ Value: 6.00
Abstract provided by Publisher
Since listeriosis, caused by Listeria monocytogenes, is one of the important concerns of public health in Europe related to foodborne zoonoses, an efficient protocol for isolate typing is necessary when performing epidemiological studies. Three standardized PFGE protocols available for L. monocytogenes were briefly reviewed. Since observing a poor-quality of ApaI pulsotypes in our laboratory, enzymes from three different manufacturers were compared. The obtained pulsotypes showed that restriction digestion with ApaI from New England BioLabs should be complemented with a subsequent overnight incubation of PFGE plugs in TE buffer for better performance.