Possibilities in identification of genomic species of Burkholderia cepacia complex by PCR and RFLP

Lucie Navrátilová, Magdaléna Chromá, Vojtěch Hanulík, Vladislav Raclavský
Pol J Microbiol
2013; 62 (4):
ICID: 1087839
Article type: Original article
IC™ Value: 10.00
Abstract provided by Publisher
 
The strains belonging to Burkholderia cepacia complex are important opportunistic pathogens in immunocompromised patients and cause serious diseases. It is possible to get isolates from soil, water, plants and human samples. Taxonomy of this group is difficult. Burkholderia cepacia complex consists of seventeen genomic species and the genetic scheme is based on recA gene. Commonly, first five genomovars are occurred in humans, mostly genomovars II and III, subdivision IIIA. Within this study was tested identification of first five genomovars by PCR with following melting analysis and RFLP. The experiments were aimed on eubacterial 16S rDNA and specific gene recA, which allowed identification of all five genomovars. RecA gene was appeared as more suitable than 16S rDNA, which enabled identify directly only genomovars II and V, genomovar I, III and IV were similar within 16S rDNA sequence.
PMID 24730131 - click here to show this article in PubMed
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