Characterization of Klebsiella pneumoniae strains isolated from urinary tract infections: Detection of ESBL characteristics, antibiotic susceptibility and RAPD genotyping
Mustafa Onur Aladag, Ahmet Uysal, Niyazi Dundar, Yusuf Durak, Erdogan Gunes Pol J Microbiol 2013; 62 (4): ICID: 1087843 Article type: Original article IC™ Value: 10.00
Abstract provided by Publisher
In this study a hundred Klebsiella pneumoniae strains isolated from urinary tract infections were evaluated in terms of genotyping, susceptibility to certain antibiotics and detection of extended spectrum of beta lactamase (ESBL) production. Random amplified polymorphic DNA (RAPD-PCR) method was used to identify the genetic differentiation of K. pneumoniae isolates. A total of 26 different DNA bands ranging between 334 bp and 28033 bp were determined among the strains. It was found that 100 K. pneumoniae strains revealed 11 different RAPD profiles. Antibiotic susceptibility tests conducted by using a disc diffusion method against 16 antibiotics exhibited that the strains showed 55 different types of resistance profiles. ESBL productions of the strains were determined by double disc synergy test (DDST) and ESBL E-test methods. ESBL production rates were found as 55% by E-test method and 45% by DDST methods among the strains. While ESBL producing K. pneumoniae strains showed the greatest resistance to penicillin G (100%), followed by piperacillin (92.7%) and erythromycin (85.4%); resistance rates of ESBL non-producing strains were determined as 97.8%, 88.8% and 88.8% to those antibiotics, respectively. Both two group strains were showed the highest sensitivity to meropenem. Based on the results obtained from study it was concluded that detection of ESBL producing strains by E-test method was more sensitive than DDST method. Phenotypic and genotypic identification methods should be used together to detection of ESBL presence. RAPD-PCR method only will not be adequate in genotyping of the strains and alternative DNA based methods should be used.