Phylogenetic and biochemical characterization of a new halo-thermotolerant, biofilm-forming Bacillus from Saline Lake of Iran
Azam Safary, Rezvan Moniri, Seyyed Mehdi Mirhashemi, Hossein Nikzad, Mostafa Akbarzadeh khiavi Pol J Microbiol 2013; 62 (4): ICID: 1087845 Article type: Original article IC™ Value: 10.00
Abstract provided by Publisher
Molecular and microbiological researches have revealed the presence of moderately to extremely halophilic microorganisms in saline environments. In this study, five halotolerant Bacillus isolated from Aran-Bidgol Saline Lake in Iran. Screening bacteria led to the identification of a unique halo-thermotolerant Bacillus. On the basis of genetic and phenotypic data, this isolate was closely related to Bacillus licheniformis. But isolated Bacillus can be distinguished from B. licheniformis by salt tolerance, 16S rDNA sequence and some different physic-chemical properties. Thus, suggested that the isolate was not the known Bacillus. Optical density of eluted stain in 590 nm indicated strong biofilm formation (OD590 1.25 ± 0.31) for this Bacillus. Also this isolate exhibited average tolerant to 1-25 mM concentrations of zinc and was sensitive to all concentrations of nickel. In biosurfactant production assay, this Bacillus exhibited the highest activity for semi-quantities oil displacement test (3.14 ± 0.02 cm2) and evaluated positive for drop-collapse test and hemolytic activity. Moreover, amylase, protease and DNAase enzymes produced in present of 10-20% salt of medium. Therefore, identified Bacillus could supply potential microbial materials for bioremediation purposes and biotechnology applications.