Application of a real-time PCR method for Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens detection in water samples
Beata Rozwadowska, Marta Albertyńska, Grzegorz Hudzik, Hubert Okła, Krzysztof P. Jasik, Jan Słodki Pol J Microbiol 2013; 62 (4): ICID: 1087848 Article type: Short communication IC™ Value: 6.00
Abstract provided by Publisher
The aim of this study was to determine the effectiveness and usefulness of a real-time PCR method, when compared to the recommended bacteria culture method, in diagnostics of pathogens in water samples. The research concerned the detection and identification of main sanitary indicators of water such as: Salmonellaspp., Escherichia. coli, Staphylococcus aureus and Clostridium perfringens. The analyses were conducted in water samples contaminated with the reference material (the aforementioned bacteria) and real environmental samples, which were examined for the presence of nucleic acid of: Salmonellaspp., E. coli, S. aureus and C. perfringens using a real-time PCR method.