Uropathogenic Escherichia coli Isolates with Different Virulence Genes Content Exhibit Similar Pathologic Influence on Vero Cells
Jamil M.A.S Obaid, Samira R. Mansour, Mohammed S. Elshahedy, Tarik E. Rabie, Adel M.H. Azab Pol J Microbiol 2014; 63 (1): ICID: 1115075 Article type: Original article IC™ Value: 10.00
Abstract provided by Publisher
UropathogenicEscherichia coli are the major causative agent of urinary tract infection -theymay simultaneously express a number of virulencefactors to cause disease. The aim of this study wasto investigate the relation between virulencefactors content of fifteen UPEC isolates andtheir pathogenic potential. The isolates belonged to the five serotypes O78:K80, O114:K90, O142:K86, O164 and O157. Nine of the virulencefactors have been explored, ibeA, pap, sfa/foc, cnf1, hly, fyuA, pil, ompT and traT. Virulencefactors profiling of the isolates revealed a different content rangingfrom 22% to 100% of the virulencegenes explored. The pathogenic capacity of all fifteen isolates when tested on Vero cells showed that the cytotoxicity for all tested strains on Vero cells was approximately equal and enhanced after growth in syncase broth, leading mainly to cell lysis.Thetoxic effects reduced slightly after heat treatment of the toxin, and greatly after formalin detoxification, but not all the deleterious effect was abolished. Endotoxin also has cytotoxic effects on Vero cells, but longer time is needed for cytolysis which isgreatly diminished with formalin treatment. In conclusion, our study revealed that pathogenic strains of UPEC can exert their pathogenic effect on live cells or system with limited virulencefactors gene content.