Novobiocin Sensitivity of Salmonella typhimurium dam and/or seqA Mutants

Abdelwaheb Chatti , Meriem Aloui , Jihen Tagourti , Mouadh Mihoub , Ahmed Landoulsi
Pol J Microbiol
2014; 63 (1):
ICID: 1115076
Article type: Original article
IC™ Value: 10.00
Abstract provided by Publisher
This study was carried out to determine the effects of novobiocin, a gyrase inhibitor, on the growth, the survival, the motility and the whole cell proteins of S. Typhimurium dam and/or seqA strains. Our results showed that the dam and seqA/dam mutants are the most sensitive to novobiocin, compared to wild type and seqA strains. Surprisingly, the motility of seqA mutants increased after exposure to novobiocin only in stationary phase cells. All of other strains showed a significant decrease in their motility. The analysis of protein profiles of all strains demonstrated several modifications as manifested by the alteration of the expression levels of certain bands. Our work is therefore of a great interest in understanding the effects of novobiocin on S. Typhimurium and the involvement of DNA methylation.

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