The Utility of Caco-2 cells in Isolation of Enteroviruses from Environmental and Clinical Material.

Magdalena Wieczorek, Agnieszka Ciąćka, Agnieszka Witek, Bogumiła Litwińska
Pol J Microbiol
2014; 63 (1):
ICID: 1115079
Article type: Original article
IC™ Value: 10.00
Abstract provided by Publisher
 
The work presented here demonstrates the utility of Caco-2 cells in isolation of enteroviruses (EVs) from environmental and clinical materials. Thirty-two samples of cerebrospinal fluid positive in Pan-entero RT-PCR were put to EV strain isolation in cell culture. Out of the 32 samples analysed, 22 (68.75%) were positive for enteroviruses by isolation in Caco-2 cells, and 10 (31.25%) were positive by isolation in RD cells. High viral titre in clinical specimens resulted in rate increase for isolation in Caco-2 cells and RD cells (87.5% and 50%, respectively). Also, the probability of isolation of enteroviruses from sewage in Caco-2 cells was 20 times higher that in RD cells. We proved that Caco-2 cells were more effective than RD cells in enterovirus isolation, irrespective of the material used in the inoculation process.
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