Detection of Sulfonamide Resistance Genes via in situ PCR-FISH

Anna Gnida, Katarzyna Kunda, Aleksandra Ziembińska, Aneta Łuczkiewicz, Ewa Felis, Joanna Surmacz-Górska
Pol J Microbiol
2014; 63 (2):
ICID: 1115551
Article type: Original article
IC™ Value: 10.00
Abstract provided by Publisher
Due to therising use of antibiotics and as a consequence of theirconcentration in the environment an increasing number of antibiotic resistant bacteria is observed. The phenomenon has a hazardous impact onhuman and animal life. Sulfamethoxazole is one of the sulfonamides commonly detected in surface waters and soil. The aim of the study wasto detect sulfamethoxazole resistance genes in activated sludge biocenosis by use of in situ PCR and/or hybridization.So far no FISH probes for the detection of SMX resistance genes have beendescribed in the literature. We have tested common PCR primers used for SMX resistance genes detection as FISH probes as well as a combination of in situ PCR and FISH. Despite thepresence of SMX resistance genes in activated sludge confirmed via traditional PCR, thedetection of the genes via microscopic visualization failed.

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