Bioprotective Potential of Bacteriocinogenic Enterococcus gallinarum Strains
Isolated from Some Nigerian Fermented Foods, and of Their Bacteriocins
Iyabo C. Oladipo, Abiodun I. Sanni, Writachit Chakraborty, Somnath Chakravorty, Sayantan Jana, Deep S. Rudra, Ratan Gacchui, Snehasiktas Swarnakar Pol J Microbiol 2014; 63 (4): ICID: 1130752 Article type: Original article IC™ Value: 10.00
Abstract provided by Publisher
Enterococcus gallinarum strains isolated from some Nigerian fermented foods were found to produce bacteriocins. The bacteriocins had a broad spectrum of activity against both Gram positive and negative bacteria. The effects of the bacteriocins and bacteriocinogenic organisms on Staphylococcus aureus infections in rats were evaluated. Sprague-Dawley rats were infected with S. aureus MTCC 737 and treated with E. gallinarum T71 and different concentrations of the bacteriocins from E. gallinarum W211 and T71. Staphylococcus aureus infection caused significant upregulation of aspartate aminotransferase and alanine aminotransferase levels in sera of the infected rats. Moreover, gelatin zymography revealed that infected gastric tissues showed elevated matrix metalloproteinase-9 activity. Bacteriocin treatments reduced the MMP-9 activity and inhibited the expressions of both Tumour Necrosis Factor Alpha (TNF-α) and Interleukin-1 Beta (IL-1β), pointing to a potential role of the bacteriocins in attenuating inflammatory responses to Staphylococcus aureus infection. Gastric and GIT damage caused by Staphylococcal infection were reduced in the Enterococcus gallinarum T71 and bacteriocin-treated groups. We conclude that these bacteriocins may have useful biomedical applications.