Antiquorum from Freshwater Zoosporic Isolated Fungi: Maximization of Some Ameliorative Factors
Essam Ahmed, Mohamed I. F. Ammar Pol J Microbiol 2014; 63 (4): ICID: 1130754 Article type: Original article IC™ Value: 10.00
Abstract provided by Publisher
Many bacteria detect their critical cell numbers through a mechanism known as Quorum sensing (QS). Acyl homoserine lactone (AHL) mediated QS systems have been shown to operate in important human pathogens. The aim of this study is to evaluate the inhibitory effect produced by zoosporic freshwater fungi on AHLs production. A potent strain of zoosporic fungi, Leptolegniella keratinophilum, was isolated after extended screening study. A new cheap medium was used to support the anti-AHLs activities. Statistical strategy depending on two levels factorial model and three levels quadratic model were applied to guide a production maximization process. The three levels Box-Behnken model possessed the ability to optimize the significant nutritional and cultural conditions and to predict the maximal that can be achieved. The study started by anti-AHLs activities 63% and reached about 88% at the end of the study. No reported exploration for such activities in these zoosporic fungi, which can make it a novel trend. Moreover, there is no previous maximization methodology for neither the experimental medium nor the fungi. The results suggest freshwater zoosporic fungi as a prospective source against bacterial infections.