Identification of Some Yeasts by Fatty Acid Profiles

Bassam Aloklah, Anwar Alhajali, Sabah Yaziji
Pol J Microbiol
2014; 63 (4):
ICID: 1130759
Article type: Short communication
IC™ Value: 6.00
Abstract provided by Publisher
One hundred and seventy eight isolates yeast species were identified using ID 32 C strip from various foods;  Saccharomyces cerevisiae(47), Rodotorula mucilaginosa (19), Candida Kefyr (33), Candida Krusei (37), Candida Lusitanie (28), Candida Lipolytica (14). Yeasts were harvested, saponified, derivatized, and extracted, and fatty acid analysis was performed by Gas Chromatography Mass Spectrometry. Results revealed  that the yeast species contained Decanoic acid (C10:0), Luaric acid (C12:0), Myristoleic acid (C14:1), Myristic acid (C14:0), Pentadecanoic acid (C15:0), Palmitoleic acid C16:1), Pamitic acid (C16:0), Heptadecenoic acid (C17:1), Linoleic acid (C18:2), Linolenic acid (C18:3), Oleic acid (C18:1) and Stearic acid (C 18:0). The differences of these fatty acids composition could be use to identify this species with an accurate, efficient and relatively rapid method.

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