Mehmet Burcin Mutlu, Kıymet Güven Pol J Microbiol 2015; 64 (1): ICID: 1148773 Article type: Original article IC™ Value: 10.00
Abstract provided by Publisher
A combination of culture-dependent and culture-independent approaches were employed to identify bacterial diversity of Çamaltı solar saltern in Turkey. The bacterial populations of Çamaltı Saltern were analyzed by molecular techniques that included denaturing gradient gel electrophoresis of 16S rRNA gene fragments PCR amplified from DNA extracted from the saltern and 16S rRNA gene library analysis.
A total of 42 isolates were identified at the genus/species level and 17 of them were found to be belonging to the Bacteria domain. All bacterial isolates were phylogenetically related to Halobacillus, Virgibacillus and Halomonas genus. A total of 50 clones from 16S rRNA gene library were analyzed by ARDRA and most (85%) of the bacterial clones were related to Salinibacter genus members of the Bacteroidetes. The sequences of DGGE bands were related to the Uncultured Salinibacter, Uncultured Halophilic Bacterium and Halomonas sp. This work highlights the halophilic bacterial diversity of Çamaltı marine solar saltern.