The Application of Impedance Microsensors for Real-Time Analysis of Pseudomonas aeruginosa Biofilm Formation

Konrad Chabowski, Adam Feliks Junka, Patrycja Szymczyk, Tomasz Piasecki, Andrzej Sierakowski, Beata Maczynska, Karol Nitsch
Pol J Microbiol
2015; 64 (2):
ICID: 1160941
Article type: Original article
IC™ Value: 10.00
Abstract provided by Publisher
Biofilms formed by nosocomial pathogens represent a major threat to patients undergoing invasive procedures. As prophylaxis remains the most efficient anti-biofilm option, it is of paramount importance to develop diagnostic tools able to detect biofilm at the early stage of formation. The present study investigates the ability of impedance microsensors to detect Pseudomonas aeruginosa biofilm presence using the impedance spectroscopy method. The measured data were analyzed using Electrical Equivalent Circuit modelling (EEC). It allowed to recognize conduction and polarization phenomena on the sensors surface and in its environment. The impedance assay results, confirmed by means of electron microscopy and quantitative cultures, indicate that specific EEC parameters may be used for monitoring the development of pseudomonal biofilm.

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