Bloodstream Infections due to Enterobacteriacae Among Neonates in Poland – Molecular Analysis of the Isolates
Agnieszka Chmielarczyk, Monika Pobiega, Jadwiga Wojkowska-Mach, Dorota Romaniszyn, Piotr B. Heczko, Malgorzata Bulanda Pol J Microbiol 2015; 64 (3): ICID: 1170461 Article type: Original article IC™ Value: 10.00
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Introduction: Bloodstream infections (BSIs) are associated with a significantly increased risk of fatality. No report has been found about the molecular epidemiology of Enterobacteriaceae causing BSI in neonates in Poland. The aim of this work was to determine the antibiotic resistance profiles, virulence gene prevalence, the epidemiological and genetic relationships among the isolates from Enterobacteriaceae causing BSI in neonates with birth weight <1501g. Methods: Antimicrobial susceptibility testing was performed. PCR was performed to identify the presence of common beta-lactamase genes, virulence genes. PFGE and MLST were performed. Results: The surveillance group contained 1,695 newborns. The incidence rate for BSIs was 5.9%, the fatality rate 15%. The most common species were E. coli (n=24) and K. pneumoniae (n=16). The CTX-M-15 was found in 15 isolates. Among E. colifimH (83.3%), ibeA (37.5%), neuC (20.8%) were most frequent. PFGE demonstrated unique pulsotypes among E.coli. ST131 was found in 7 isolates. PFGE of 16 K. pneumoniae strains showed 8 pulsotypes. Five isolates from one NICU belonged to one clone. MLST typing revealed 7 different ST with ST336 as the most prevalent. Conclusion: This study provides additional information about Enterobacteriaceae causing BSI among neonates. E. coli and Klebsiella spp. isolated in this study have completely different epidemiology to each other.