Molecular methods for identification of monophasic Salmonella Typhimurium strains
Grzegorz Madajczak, Bożena Dera-Tomaszewska, Jolanta Szych, Anna Chróst, Monika Wasiak Pol J Microbiol 2015; 64 (4): ICID: 1185238 Article type: Short communication IC™ Value: 6.00
Abstract provided by Publisher
Two molecular biology methods were used to differentiate Salmonella enterica 1,4,,12:i:- strains: “Salmonella Check&Trace microarray” (CT) and multiplex PCR (mPCR). For 92 strains in CT result “Salmonella 1,4,,12:i:-” were obtained. Those strains were confirmed in mPCR as monophasic fljB-lack Salmonella Typhimurium. For 17 strains, which in CT assay were recognized as Salmonella Typhimurium, the same identification was obtained in mPCR. Reference Salmonella strains: Lagos, Agama, Tsevie, Glocester and Tumodi in CT were recognized as Salmonella genovar, in mPCR – as Salmonella O:4, H:i other than Salmonella Typhimurium, the same like Salmonella Farsta, recognized incorrectly in CT as Salmonella Typhimurium.