Detection of Polioviruses in Sewage Using Cell Culture and Molecular Methods

Agnieszka Figas, Magdalena Wieczorek, Bogumiła Litwińska, Włodzimierz Gut
Pol J Microbiol
2016; 65 (4):
ICID: 1227676
Article type: Short communication
IC™ Value: 6.00
Abstract provided by Publisher
 
The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures.
DOI: 10.5604/17331331.1227676
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