Detection of Polioviruses in Sewage Using Cell Culture and Molecular Methods
Agnieszka Figas, Magdalena Wieczorek, Bogumiła Litwińska, Włodzimierz Gut Pol J Microbiol 2016; 65 (4): ICID: 1227676 Article type: Short communication IC™ Value: 6.00
Abstract provided by Publisher
The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures.