An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices

Angélica Godínez-Oviedo, Gerardo M. Nava, Sofía M. Arvizu-Medrano, Montserrat Hernández-Iturriaga
Pol J Microbiol
2017; 66 (2):
ICID: 1242033
Article type: Short communication
IC™ Value: 6.00
Abstract provided by Publisher
 
Several studies have observed that a conventional PCR protocol using primers LM1 and LM2 for the identification of gene hlyA Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason in this study, we provide a modified PCR protocol that improves the specificity of the LM1 and LM2 primers
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