Detection of Enterotoxic Bacillus cereus Producing Hemolytic and Non Hemolytic Enterotoxins by PCR Test

Elzbieta Oltuszak-Walczak, Piotr Walczak, Robert Modrak
Pol J Microbiol
2006; 55 (2):
ICID: 477450
Article type: Original article
IC™ Value: 9.00
Abstract provided by Publisher
 
Nine strains belonging to Bacillus cereus group has been isolated from food and environmental samples. Their taxonomic position was confirmed by RFLP analysis of 16S rRNA gene digested with TaqI. The detection of DNA sequences encoding the hemolysin BL complex and enterotoxin NHE, was studied in Bacillus sp. isolates. Set of primers was used to amplify fragment of hblD gene by PCR. For the detection of nheB gene a new primer set was developd which allowed to amplify 273 bp fragment from wide number of strains belonging to Bacillus cereus group. The hblD gene was present in 7 out of 9 isolates whereas nheB gene occurred in all of them. Reference strains of B. cereus LOCK0807, and B. thuringiensis NCAIM 01262 contained both genes. Strains of Bacillus subtilis ATCC 6633 and B. pumilus LOCK 0814 do not contain both genes. Obtained results showed that B. thuringiensis NCAIM 01262 contains both genes and therefore may be harmful for human beings.
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